Journal: Journal of vascular research

Article Title: Microvascular endothelial cell responses in vitro and in vivo: modulation by zoledronic acid and paclitaxel?

doi: 10.1159/000313876

Figure Lengend Snippet: Fig. 7. The effect of treatment with zole- dronic acid or paclitaxel on spleen vascu- lature. CD1 nude mice were treated with saline, 100 g/kg i.p. zoledronic acid 20 mg/kg i.v. paclitaxel, alone or in combina- tion. Animals were sacrificed and the spleen isolated and processed for histolog- ical analysis of the vasculature following staining with an antibody to CD34. a Con- trol. b Paclitaxel only. c Zoledronic acid only. d Paclitaxel and zoledronic acid.

Article Snippet: Sections were stained using a rat antibody specific for mouse CD34 (MCA1825CA 1: 50, ABDSerotec, UK) and an IgG2A isotype control, EC and vessel morphology were assessed by an experienced histopathologist (S.S.C.) who was blinded to the treatment groups.

Techniques: Saline, Isolation, Staining

Journal: Scientific Reports

Article Title: Immunohistochemical properties of embryonic telocytes in a myogenic microenvironment

doi: 10.1038/s41598-024-62103-1

Figure Lengend Snippet: Identification of telocytes in the developing muscles using CD34. Paraffin sections immuneostained for CD34. CD34-positive telocytes (arrows) formed 3D network within the developing muscles. Note nascent myotube (m).

Article Snippet: CD34 , MOUSE ANTI CHICKEN CD34 (Bio rad) , MOUSE ANTI CHICKEN CD34 Monoclonal Antibody (Clone: AV138) (Cat.no MBS224490) , 1:100 , Over night , Boiling in citrate buffer (pH 6.0), 20 min , Goat anti-Mouse IgG (H + L) Secondary Antibody Catalog # 31569 Dilution; 1:100 1 h at room temperature.

Techniques: Muscles

Journal: Scientific Reports

Article Title: Immunohistochemical properties of embryonic telocytes in a myogenic microenvironment

doi: 10.1038/s41598-024-62103-1

Figure Lengend Snippet: A column graphic representation of the mean numbers of positive CD34, VEGF, CD68, CD21 receptor TCs at 8 days embryo. Data are presented as the mean ± standard error (n = 8). IHC Expression with different lowercase letters are significantly different (one-way ANOVA: p < 0.05).

Article Snippet: CD34 , MOUSE ANTI CHICKEN CD34 (Bio rad) , MOUSE ANTI CHICKEN CD34 Monoclonal Antibody (Clone: AV138) (Cat.no MBS224490) , 1:100 , Over night , Boiling in citrate buffer (pH 6.0), 20 min , Goat anti-Mouse IgG (H + L) Secondary Antibody Catalog # 31569 Dilution; 1:100 1 h at room temperature.

Techniques: Expressing

Journal: Scientific Reports

Article Title: Immunohistochemical properties of embryonic telocytes in a myogenic microenvironment

doi: 10.1038/s41598-024-62103-1

Figure Lengend Snippet: Identity, sources, and working dilution of antibodies used in Immunohistochemical studies.

Article Snippet: CD34 , MOUSE ANTI CHICKEN CD34 (Bio rad) , MOUSE ANTI CHICKEN CD34 Monoclonal Antibody (Clone: AV138) (Cat.no MBS224490) , 1:100 , Over night , Boiling in citrate buffer (pH 6.0), 20 min , Goat anti-Mouse IgG (H + L) Secondary Antibody Catalog # 31569 Dilution; 1:100 1 h at room temperature.

Techniques: Immunohistochemical staining, Incubation, Marker, Polymer

Journal: Journal of Veterinary Science

Article Title: Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury

doi: 10.4142/jvs.2009.10.4.273

Figure Lengend Snippet: Green fluorescence protein (GFP) labeled canine adipose-derived stem cells (ASCs). (A) Typical morphological feature of canine ASCs. (B) Green fluorescence was identified in ASCs at 48 h after transfection. ×100.

Article Snippet: Thus, 30 μL each of a prediluted PE-conjugated mouse anti-dog CD14 (#CAM46A), a PE-conjugated mouse anti-dog CD34 (MCA2411F; AbD Serotec, USA), a PE-conjugated mouse anti-dog CD45-like (CADO18A), a PE-conjugated rat anti-dog CD44 (ab19622; Abcam, UK), a PE-conjugated mouse anti-dog CD90 (DH2A) and a FITC-conjugated mouse anti-human CD105 (555690; BD Biosciences, USA) antibody was used in individual test.

Techniques: Fluorescence, Labeling, Derivative Assay, Transfection

Journal: Journal of Veterinary Science

Article Title: Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury

doi: 10.4142/jvs.2009.10.4.273

Figure Lengend Snippet: Adipogenic and osteogenic differentiation of canine adipose-derived stem cells (ASCs). A: ASCs cultured in DMEM + 10% FBS media (control media), not stained by Oil red O. B: Oil red O stained after 3 weeks incubation at adipogenic media. C: ASCs cultured in control media, not stained with Alizarin red S. D: Intense Alizarin red S stained after 3 weeks incubation at osteogenic media and confirmed calcium deposition. A and B: Oil red O stain, C and D: Alizarin red S stain, ×100.

Article Snippet: Thus, 30 μL each of a prediluted PE-conjugated mouse anti-dog CD14 (#CAM46A), a PE-conjugated mouse anti-dog CD34 (MCA2411F; AbD Serotec, USA), a PE-conjugated mouse anti-dog CD45-like (CADO18A), a PE-conjugated rat anti-dog CD44 (ab19622; Abcam, UK), a PE-conjugated mouse anti-dog CD90 (DH2A) and a FITC-conjugated mouse anti-human CD105 (555690; BD Biosciences, USA) antibody was used in individual test.

Techniques: Derivative Assay, Cell Culture, Control, Staining, Incubation

Journal: Journal of Veterinary Science

Article Title: Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury

doi: 10.4142/jvs.2009.10.4.273

Figure Lengend Snippet: Flow cytometric analysis of surface-marker expression on ASC. The seventh passage of ASCs expressed CD44, CD90 and CD105, and were negative for CD14, CD34, CD45. The overwhelming majority (> 95%) of cASC expressed the mesenchymal cell surface markers CD90 and CD105.

Article Snippet: Thus, 30 μL each of a prediluted PE-conjugated mouse anti-dog CD14 (#CAM46A), a PE-conjugated mouse anti-dog CD34 (MCA2411F; AbD Serotec, USA), a PE-conjugated mouse anti-dog CD45-like (CADO18A), a PE-conjugated rat anti-dog CD44 (ab19622; Abcam, UK), a PE-conjugated mouse anti-dog CD90 (DH2A) and a FITC-conjugated mouse anti-human CD105 (555690; BD Biosciences, USA) antibody was used in individual test.

Techniques: Marker, Expressing

Journal: Journal of Veterinary Science

Article Title: Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury

doi: 10.4142/jvs.2009.10.4.273

Figure Lengend Snippet: Immunofluorescence staining of ASC group; A1-A3: Glial fibrillary acidic protein (GFAP), B1-B3: Neurofilament M (NF160), C1-C3: Neuronal class III beta tubulin (Tuj-1), D1-D3: Oligodendrocyte, Green fluorescence protein (GFP)- labeled lentiviral vector inserted stem cells were positive with GFAP, NF 160, Tuj-1, and oligodendrocytes in injured lesions (arrows). Scale bars = 50 µm.

Article Snippet: Thus, 30 μL each of a prediluted PE-conjugated mouse anti-dog CD14 (#CAM46A), a PE-conjugated mouse anti-dog CD34 (MCA2411F; AbD Serotec, USA), a PE-conjugated mouse anti-dog CD45-like (CADO18A), a PE-conjugated rat anti-dog CD44 (ab19622; Abcam, UK), a PE-conjugated mouse anti-dog CD90 (DH2A) and a FITC-conjugated mouse anti-human CD105 (555690; BD Biosciences, USA) antibody was used in individual test.

Techniques: Immunofluorescence, Staining, Fluorescence, Labeling, Plasmid Preparation

Journal: Cells

Article Title: IMG-A1: A Novel Immortalized Granulosa Cell Line for Investigating FSH-Dependent Folliculogenesis and Ovarian Pathophysiology

doi: 10.3390/cells14241940

Figure Lengend Snippet: Basic Characterization of the IMG-A1 Granulosa Cell Line. ( a , b ) Phase-contrast micrographs of the IMG-A1 cell culture. ( c ) Staining for senescence-associated β-galactosidase activity in passage 18 cells. ( d – f ) Immunocytochemical staining for the proliferation marker Ki67, showing a phase-contrast image ( d ), Ki67 immunofluorescence ( e ), and a merged image with DAPI nuclear counterstain ( f ). ( g , h ) Representative metaphase spreads showing a normal diploid karyotype (40 chromosomes) ( g ) and a near-tetraploid karyotype (78 chromosomes) from the IMG-A1 line ( h ). ( i – k ) Ploidy analysis by flow cytometry. Control blood cells ( i ) and primary granulosa cells ( j ) show characteristic diploid (2n) and tetraploid (4n, G2/M phase) peaks. The IMG-A1 culture ( k ) displays a predominantly near-tetraploid and near-octaploid cell population. ( l ) Flow cytometry analysis confirming the homogeneity of the IMG-A1 culture (passages 10 and 40) based on staining for the stromal marker CD29 and the absence of hematopoietic markers CD45, CD34, and the fibroblast marker CD90. Quadrant gates were set based on unstained controls. Scale bars = 100 µm.

Article Snippet: PE CD34 Rat mAb [RAM34] , Elabscience Biotechnology Co., Ltd., Wuhan, China , E-AB-F1284D.

Techniques: Cell Culture, Staining, Activity Assay, Marker, Immunofluorescence, Flow Cytometry, Control